Topics in Particle and Dispersion Science

In situ marine flow-cytometers

Prev topic | Next topic

In situ particle counting and characterization avoids problems caused by sampling and sample handling in aquatic sciences. However, the design of in situ instruments for particle characterization, generally referred to as flow-cytometers (FCM) (for example, Dubelaar GBJ and Jonker 2000) is more complicated than that of lab flow-cytometers (for example, Davey HM and Kell 1996). Traditional designs which define the sample volume by drawing the sample through a narrow channel must address the problem of clogging of this channel by oversized particles (for example, Olson RJ et al 2003, Dubelaar GBJ and Gerritzen 2000).

Attempts have been made in the past to circumvent this problem by defining the sample volume optically (for example, Wang JCF and Hencken 1986, who used two color lasers to define nested sensing zones). Wang X et al (2005) present a novel underwater FCM that also defines the sample volume by optical means: the crossed beams of a laser Doppler anemometer. Signal thresholding allows one to ignore particles skimming the edges of this volume.

CITATION: Jonasz M. 2006. In situ marine flow-cytometers (www.tpdsci.com/Tpc/FCMInSitu.php). In: Top. Part. Disp. Sci. (www.tpdsci.com).

HISTORY: Published: 13-Jan-2006 Modified: 02-Jul-2006 Peer-reviewed: PENDING

Entire site Survey Topics Index References
Copyright 2005-2012 TPDSci Inc. All rights reserved. | Terms of use