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In situ particle counting and characterization avoids problems caused by sampling and sample handling in aquatic sciences. However, the design of in situ instruments for particle characterization, generally referred to as flow-cytometers (FCM) (for example, Dubelaar and Jonker 2000) is more complicated than that of lab flow-cytometers (for example, Davey and Kell 1996). Traditional designs which define the sample volume by drawing the sample through a narrow channel must address the problem of clogging of this channel by oversized particles (for example, Olson et al 2003, Dubelaar and Gerritzen 2000).
Attempts have been made in the past to circumvent this problem by defining the sample volume optically (for example, Wang and Hencken 1986, who used two color lasers to define nested sensing zones). Wang et al (2005) present a novel underwater FCM that also defines the sample volume by optical means: the crossed beams of a laser Doppler anemometer. Signal thresholding allows one to ignore particles skimming the edges of this volume.
| CITATION: Jonasz M. 2006. In situ marine flow-cytometers (www.tpdsci.com/Tpc/FCMInSitu.php). In: Top. Part. Disp. Sci. (www.tpdsci.com). |
HISTORY: Published: 13-Jan-2006 Modified: 02-Jul-2006 Peer-reviewed: PENDING |
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